Journal of Carcinogenesis & Mutagenesis

Division of Applied Life Sciences, Graduate School of Engineering, Sojo University, Kumamoto, Japan

Publications

• Research Article   
  Hybrid Liposomes of Nanoparticles Achieve Targeted Accumulation and Induce Apoptosis in TE-4 Esophageal Cancer Cells Without Antitumor Drugs
  Author(s): Junna Takai, Masaki Okumura, Koichi Goto, Yoko Matsumoto and Hideki Ichihara*
  
  Nanoparticle Hybrid Liposomes (HL), composed of L-α-dimyristoylphosphatidylcholine (DMPC) vesicles and polyoxyethylene (25) dodecyl ether (C12 (EO)25 ) micelles, were prepared by ultrasonic irradiation in a 5% glucose solution. The HL remained stable for over one month, maintaining a hydrodynamic diameter below 100 nm. HL exhibited significant growth-inhibitory effects on esophageal cancer (TE-4) cells in the absence of antitumor drugs, as determined by the WST-8 assay. Flow cytometric analysis revealed an increase in apoptotic cells following HL treatment. Fluorescent substrate-based assays further demonstrated activation of caspase-3, -8, and -9, indicating that HL induced apoptosis via a caspase-dependent pathway in TE-4 cells. Accumulation of HL containing fluorescent probe (1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-glycero-3-phosphocholine (NBDP.. View more»
  

  DOI: 10.35248/2157-2518.25.16.490

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