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Delimaris Ioannis
Department of Medicine, Center for Instruction, Research, and Technology, Metropolitan College, Athens, Greece
Publications
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Short Communication
Current Knowledge Regarding the Biological Methods of Assessing DNA Damage in Humans
Author(s): Delimaris Ioannis*
This work summarizes the information available currently regarding the biological methods of assessing DNA (Deoxyribonucleic Acid) damage in humans. The methods include the comet assay (for determining single-strand DNA breaks, alkali-labile sites, and cross-links), the micronucleus assay, cytogenetic assays (which include the sister chromatid exchange assay and chromosomal aberration assay), oxidative DNA damage assays (including the measurement of oxidized bases like 8-oxo-7, 8-dihydro-2'-deoxyguanosine), and DNA repair assays. However, selecting the best method will depend on a number of factors, including the study's objectives, the type of DNA damage being measured, the resources at hand, and the study's size. A more comprehensive understanding of DNA damage and its potential effects on human health may also result from combining different techniques. Assay s.. View more»