Journal of Clinical & Experimental Pharmacology

Silvia De Francia

Publications

• Research Article
  A New HPLC Validated Method for Therapeutic Monitoring Of Triazoles in Human Plasma: First Results in Leukaemic Patients
  Author(s): Silvia De Francia, Paolo Cardalana MLT, Elisa Pirro P, Francesca Maria Piccione, Giuliana Abbadessa, Vitina Carriero, Antonia Rotolo, Marco De Gobbi, Angelo Guerrasio, Silvia Racca and Giuseppe ReimondoSilvia De Francia, Paolo Cardalana MLT, Elisa Pirro P, Francesca Maria Piccione, Giuliana Abbadessa, Vitina Carriero, Antonia Rotolo, Marco De Gobbi, Angelo Guerrasio, Silvia Racca and Giuseppe Reimondo
  
  Abstract Therapeutic drug monitoring of triazoles is widely used in clinical practice to optimize therapy, especially in those patients who need antifungal treatment as co-medications. Here it is described development and validation of a new chromatographic method in order to quantify voriconazole and posaconazole in human plasma by ultraviolet detection. After liquid extraction of analytes from plasma using acetonitrile, samples are evaporated to dryness and then reconstituted in mobile phase for chromatographic separation. Analysis is achieved on C18 reverse phase column and eluate is monitored at 250 nm. Mobile phase consisted of 35% water, 15% methanol, 50% acetonitrile. Flavone was used as internal standard; retention times (minutes) were, respectively, for voriconazole 3.9, posaconazole 7.9, flavone 7.1. Accuracy and variability were assayed by inter- and intra-day validation, c.. View More»
  DOI: 10.4172/2161-1459.1000189

  Abstract PDF