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Rania A. Ghonaim
Rania A. Ghonaim
Egypt
Publications
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Research Article
Comparison between Multiplex PCR and Phenotypic Detection Methods for Identifying AmpC B-lactamases Among Clinical Isolates of Enterobacteriaceae in Zagazig University Hospitals, Egypt
Author(s): Rania A. Ghonaim and Hanaa Abdel MoaetyRania A. Ghonaim and Hanaa Abdel Moaety
No standardized phenotypic methods for the screening and detection of plasmid-mediated AmpC enzymes are currently available, which is one of the main problems we are facing nowadays. Aim: This study aimed to evaluate the presence of AmpC β-lactamase among Enterobacteriaceae isolates separated from patients with nosocomial infections and to detect the most prevalent genetic strains in the separated isolates and evaluation of two phenotypic methods (AmpC E test and cefoxitin–cloxacillin double disc synergy test) to detect AmpC enzymes. Materials and methods: Total of 1200 gm negative isolates were screened for potential plasmid-mediated AmpC enzymes by cefoxitin disc, AmpC E test and cefoxitin–cloxacillin double disc synergy tests. The genotypic identificatio.. View More»
DOI: 10.4172/2327-5073.1000313