Pharmaceutica Analytica Acta

Pavan kumar K

Publications

• Research Article
  Quantitative Estimation of Lopinavir and Ritonavir in Tablets by RP-HPLC Method
  Author(s): Jagadeeswaran M, Gopal N, Pavan kumar K and Siva kumar TJagadeeswaran M, Gopal N, Pavan kumar K and Siva kumar T
  
  A reversed phase high-performance liquid chromatographic method was developed and validated for the quantitative determination of two antiviral drugs viz. lopinavir and ritonavir. Chromatography was carried out by gradient technique on a reversed-phase C18 Column, Phenomenex (250 x 4.6 mm, 5 μ) with mobile phase mixture of Buffer: Acetonitrile (45:55 v/v) was used as a mobile phase and the pH was adjusted into 4.5 by using with O-phosphoric acid, at a flow rate of 1.2 ml/min. The UV range was detected at 240 nm for lopinavir and ritonavir respectively. The different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2B guidelines. The linearity of the calibration curves for each analyte in the desired concentratio.. View More»
  DOI: 10.4172/2153-2435.1000160

  Abstract PDF