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Ozimek L
Ozimek L
Canada
Publications
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Research Article
Selective Removal of Phenylalanine Impurities from Commercial κ-Casein Glycomacropeptide by Anion Exchange Chromatography
Author(s): Nakano T and Ozimek LNakano T and Ozimek L
Bovine κ-casein glycomacropeptide (GMP) found in sweet whey is a 64 amino acid residue phosphorylated glycopeptide. Because it lacks aromatic amino acids including phenylalanine, GMP is thought to be an important dietary source of amino acids for patients suffering from phenylketonuria. There is, however, very little information available concerning preparation of phenylalanine-free GMP for human consumption. This study was, therefore, undertaken to remove phenylalanine containing impurities from commercially available crude GMP by anion exchange chromatography on diethylaminoethyl (DEAE)-Sephacel. The results demonstrated that phenylalanine containing proteins or peptides do not bind to the column, while most GMP accounting for 93% of total recovered sialic acid can bind to the column. The purified GMP, which accounted for average 43% of dry weight o.. View More»
DOI: 10.4172/2157-7110.1000537