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Marianne S. Brooks
Marianne S. Brooks
Canada
Publications
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Research Article
Partition of Pepsinogen from the Stomach of Red Perch (Sebastes marinus) by Aqueous Two Phase Systems: Effects of the Salt Type and Concentration
Author(s): Lisha Zhao, Suzanne M. Budge, Abdel E. Ghaly, Marianne S. Brooks and Deepika DaveLisha Zhao, Suzanne M. Budge, Abdel E. Ghaly, Marianne S. Brooks and Deepika Dave
An important acidic protease, pepsin is synthesized and secreted in the gastric membrane in an inactive state called pepsinogen (PG) and has applications in the food and manufacturing industries, collagen extraction, gelatin extraction and in regulating digestibility. Fish processing waste can be used to produce commercially valuable byproducts such as pepsinogen. In the present study, the purification of pepsinogen from the stomach of red perch using aqueous two phase systems (ATPS) formed by polyethylene glycol (PEG) and salt at 4°C was optimized. The effects of salt type (MgSO4, (NH4)2SO4, Na3C6H5O7 and K2HPO4) and concentration (6, 7, 8, 9, 10, 11, 12, 13, 15, 17, 19%) on the partitioning of PG were studied and parameters including total volume (TV), volume ratio (VR).. View More»
DOI: 10.4172/2157-7110.1000180