Fermentation Technology

Isao Yumoto

Publications

• Research Article
  High and Rapid L-lactic Acid Production by Alkaliphilic Enterococcus sp. by Adding Wheat Bran Hydrolysate
  Author(s): Kazuaki Yoshimune, Mika Yamamoto, Tomo Aoyagi and Isao YumotoKazuaki Yoshimune, Mika Yamamoto, Tomo Aoyagi and Isao Yumoto
  
  Two strains of alkaliphilic lactic acid bacteria, L-120 and AY103, which can produce L-lactic acid extensively, were isolated during trials of L-lactic acid production under unsterilized conditions at pH 9. Strains L-120 and AY103 are similar to Enterococcus casseliflavus and Enterococcus faecalis, respectively, as determined by 16S rRNA gene sequencing. It was found that wheat bran hydrolysate (1.5 or 3.0% dry w/v) strongly stimulates the production of lactic acid in both strains. Strain L-120 produced 149 g L-1 L-lactic acid at 35°C under pH 9 from 180 g L-1 glucose with the production rate of 3.9 g L-1 h-1. Strain AY103 produced 153 g L-1 L-lactic acid from 180 g L-1 glucose with the production rate of 4.2 g L-1 h-1. Both strains produced high-optical-purity (100%) L-lactic acid. In addition, strain L-120 produced 12% (w/v) L-lactic acid from degraded inedible materials, i.e., .. View More»
  DOI: 10.4172/2167-7972.1000138

  Abstract PDF