Journal of Carcinogenesis & Mutagenesis

Abstract

Therapeutic Effects of Hybrid Liposomes against Triple Negative Mouse Breast Cancer in vitro and in vivo

Hideaki Ichihara, Suguru Miyamoto, Junna Takai, Masaki Okumura, Koichi Goto, Yoko Matsumoto

Hybrid Liposomes (HL), composed of L-α-dimyristoylphosphatidylcholine (DMPC) and polyoxyethylene (25) dodecyl ether (C12(EO)25), demonstrated significant therapeutic potential against breast cancer cells. HL formed a clear and stable solution with a hydrodynamic diameter of approximately 100nm, which remained unchanged for at least four weeks, suggesting high physicochemical stability suitable for clinical application. In vitro, HL exhibited a marked antiproliferative effect on 4T1-Luc murine breast cancer cells. Furthermore, flow cytometric analysis revealed a significant increase in apoptotic cell populations following HL treatment, indicating that HL effectively induces apoptosis in breast cancer cells. In vivo, administration of HL to 4T1-Luc tumor-bearing mice resulted in a significant reduction in tumor volume and tumor weight, without any observable adverse effects or changes in body weight throughout the treatment period. These findings collectively suggest that HL exert potent growth-inhibitory effects on breast cancer both in vitro and in vivo, and may serve as a promising drug delivery system with minimal toxicity for the treatment of breast cancer.

Published Date: 2025-04-19; Received Date: 2025-03-17