Journal of Nanomedicine & Nanotechnology

Use of guar gum nanoparticle as a therapeutic agent in peritonitis

24^th World Congress on Nanomaterials and Nanotechnology

July 12-13, 2018 Bangkok, Thailand

Shinjini Mitra, Nandita Ghosh and Ena Ray Banerjee

University of Calcutta, India

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

The main aim of drug discovery is to design drugs which can target specific cells and simultaneously exert its therapeutic effect. The use of polymer based nanoparticles is useful because their functional components either helps direct the drugs to specific cells, or act as drugs themselves from within the cells. In this study, we have used a nanoparticle designed from guar gum, a gum obtained from the seeds of the leguminous plant, Cyamopsis tetragonoloba. It is a galactomannan, with mannose and galactose present in a 2:1 ratio. The Guar gum Nanoparticle (GN), of size 80 nm, was designed by the acid hydrolysis of the guar gum, and was made mannose- rich. The aim of this study was to determine the therapeutic effect of GN in vitro and in vivo in inflammatory disease. The in vitro studies performed with RAW 264.7 macrophage cells showed successful uptake of GN in a short duration of time, via their mannose receptors, as confirmed by using mannose as a competitive inhibitor. The in vitro anti-inflammatory activity of GN on RAW 264.7 cells was confirmed by Nitric Oxide (NO) and cell proliferation (MTT) assays. Since the in vitro studies showed an involvement of macrophages, we used GN in a mouse model of thioglycollate- induced peritonitis. Several assays, including flow cytometry showed a significant decrease in the inflammation with administration of GN. Thus, we concluded that GN can be successfully used as an anti- inflammatory agent for peritonitis.

Biography :

Shinjini Mitra has completed her BSc in Microbiology in 2011 and her MSc in Microbiology in 2013 from the University of Calcutta, Kolkata, India. She has joined the Immunology & Regenerative Medicine Research Laboratory of the Department of Zoology, University of Calcutta, Kolkata, in 2014 and has been working for her PhD with thesis entitled “Translational outcome research in preclinical models of inflammation and degeneration, using phytochemicals, small molecules and cell-based strategies”.

E-mail: shinjinimitra@rediffmail.com