Journal of Nanomedicine & Nanotechnology

Transient loading of hematopoietic progenitor cells by polystyrene nanoparticles is independent on their size and composition

14^th International Conference and Exhibition on Nanomedicine and Pharmaceutical Nanotechnology

April 09-11, 2018 Amsterdam, Netherlands

Bart Wathiong

Flemish Institute for Technological Research, Belgium

Scientific Tracks Abstracts: J Nanomed Nanotechnol

Abstract:

Delivery of therapeutic nanoparticles (NPs) by stem and progenitor cells can overcome the limitations of NP carriers and can create opportunities for stem cell transplantation. In this respect, we studied the interaction between engineered NPs and ex vivo derived hematopoietic progenitor cells (HPC). CD34-positive HPC were derived from human cord blood and were exposed to fluorescent carboxylate polystyrene NPs of 40, 100 and 200nm size, and to 50nm SiO2 NPs. NP uptake kinetics were assessed by measuring fluorescence intensity at short time intervals using flow cytometry. HPCs demonstrated a rapid (within 1 hour), but transient loading behavior which was retained upon changing the size and composition of the NPs. Using confocal microscopy fluorescent NPs were observed to appear mainly at the outer boundary of the cells, while only few were located in the intracellular compartment. These findings contrast with reported observations of steadily increasing NP internalization in other cell types, such as phagocytic cells or cell lines, and suggest that CD34+ progenitor cells handle NPs by using different mechanisms. Further research will be conducted to investigate the processes underlying the interactions between the NPs and the cellular membrane

Biography :

In 2014 I obtained a Master’s degree in biomedical sciences with a specialization in bio-electronics and nanotechnology at the university of Hasselt. In 2015 a started a joint-PhD between Hasselt University and VITO. VITO is a technological research institute working for the Flemish government.
Email:bart.wathiong@vito.be