Biochemistry & Analytical Biochemistry

To study allelic variants of PON1 gene in ischemic stroke patients with high LDL/HDL ratios

International Conference on Biochemistry

October 10-12, 2016 Kuala Lumpur, Malaysia

Sushree S Rautaray

Army College of Medical Sciences, India

Posters & Accepted Abstracts: Biochem Anal Biochem

Abstract:

Stroke continues to be the leading cause of morbidity and mortality worldwide. Oxidative stress is a characteristic of ischemic stroke. Elevated LDL/HDL ratio is an important factor for predicting arteriosclerosis. Paraoxonase 1(PON1) protects LDL from oxidative modifications and has a protective effect against arteriosclerosis. Two common polymorphisms, Q192R and L55M, in PON1 gene can affect PON1 levels and function. The aim of this study was to evaluate Q192R and L55M polymorphisms in ischemic stroke patients with high LDL/HDL ratios and to investigate ox-LDL levels as a marker of oxidative stress. The study included 100 patients of ischemic stroke admitted in ICU of Base Hospital, Army College of Medical Sciences, Delhi and 100 controls. Patients were in the age group of 55-85 years. PON1 Q192R and L55M were determined by PCR-RFLP method, ox-LDL by ELISA kit method. PON1 L55M was associated with high LDL/HDL ratios in ischemic stroke patients. So, the L55M polymorphism can contribute in decreasing the antioxidant function and decreasing HDL particles. Plasma levels of ox-LDL were increased in stroke patients (P<0.001) compared to controls. In conclusion, it is important to explore the effects of PON1 L55M genetic polymorphisms and the inflammatory response associated with stroke. We hypothesized that elevated ox-LDL levels and lower PON1 activity may contribute for the development of oxidative stress. The present study was carried out to emphasize the importance of these markers for early diagnosis and therapeutic interventions in ischemic stroke patients.

Biography :

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