Biology and Medicine

Study of sequences in Nicotiana genomes acquired from Agrobacterium by horizontal gene transfer

2^nd World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry

September 22-24, 2016 Phoenix, USA

Galina Khafizova

Saint-Petersburg State University, Russia

Posters & Accepted Abstracts: Biol Med (Aligarh)

Abstract:

Agrobacterium is a soil bacterium that transfer fragment of its plasmid (Ti or Ri) into plant�??s genomes; this fragment is called T-DNA. In 1983, homological to Agrobacterium sequences were found in the genome of untransformed Nicotiana glauca plant. This sequence was called cT-DNA (cellular T-DNA). Later cT-DNA was found in genomes of 14 more species of genus Nicotiana. Genus Nicotiana contains 3 subgenera. CT-DNAs in Nicotiana species inside one sub-genus were found to be more similar than cT-DNAs in species from different subgenera. It can be a result of multiple tranformation events in the evolution of genus Nicotiana. It was also shown that Nicotiana species from different sub-genus differ in their cT-DNA insertion sites, though only 2 species were analyzed. To know more about it, more species of Nicotiana need to be analyzed. For this purpose a test-system based on PCR was developed. Each system consists of 2 primers combinations. First type includes primers for plant�??s sequences, flanking T-DNA from both sides. Getting the amplicon with these primers means lack of cT-DNA in this site. Second type includes one primer for plant�??s sequence, flanking T-DNA and another one for Agrobacterium sequence close to the border of the insert, so the result of PCR is the sequence of plant-bacterium-border. Using this system for cT-DNA analysis can help to get new insights in this topic.

Biography :

Galina Khafizova was graduated from Saint-Petersburg State University in 2015. Presently she is working on her Master’s thesis in the same universit, at the Genetics and Biotechnology Department. She has participated in several conferences for young scientists with both oral and poster presentations and was also awarded for the best oral presentation. She has participated in Bioinformatic’s Summer School in July 2015 and currently she is studying bioinformatics for applying new knowledge in her research.

Email: galina.khafizova@gmail.com