Pharmaceutica Analytica Acta

Smart liposomes: Multifaceted approach to target ovarian cancer

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Ankit Jain, Sanjay K. Jain

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Recent study was aimed to evolve dual approach using synergistically active combination of paclitaxel-topotecan (Pac-Top; 20:1, w/w) using surface engineered and integrally modified liposomes. Various liposomes (size ~200 nm) viz. Liposomes (Lip), PEGylated liposomes (PL) and FR-targeted PEGylated liposomes (FPL) were developed using thin film casting technique. In vitro drug release study reflected initial burst release followed by sustained profile at physiological conditions (37?0.5?C, pH 7.4) whilst abrupt dispersal (i.e. more than 90%) within 5 min at simulated tumor conditions (41?1?C, pH 4). These liposomes were studied for shape and physical interaction (and integrity), in vitro drug release kinetics modelling, haemolytic toxicity studies, ex vivo pharmacodynamics (OVCAR-3 cell lines), florescence microscopy, and pharmacokinetics in ovarian tumorbearing mice. Potentiated anticancer activity of FPL could be attributed to multifaceted features viz. thermosenstivity, long circulatory nature and targetability.