Biochemistry & Analytical Biochemistry

Sialic acid profile and sialidase activity in HIV infected individuals

International Conference on Biochemistry

October 10-12, 2016 Kuala Lumpur, Malaysia

Hadiza Abdullahi

Northwest University Kano, Nigeria

Posters & Accepted Abstracts: Biochem Anal Biochem

Abstract:

Sialic Acids and sialidases have been implicated in many disease states particularly bacterial and viral infections which are common opportunist infections of HIV disease. A study was carried out to determine sialic acid profile and sialidase activity in HIV infected and apparently healthy individuals. Blood samples were collected from 200 subjects (150 HIV infected individuals and 50 apparently healthy individuals, divided into four groups: HIV ART naive, HIV stable (on ART but have been stable with no clinical episodes), HIV-OI (on ART with opportunistic infections), and apparently healthy). Complete blood count, erythrocyte surface sialic acid (ESSA), free serum sialic acid (FSSA) concentrations and sialidase activity were determined for all 200 subjects. Analysis of variance was used to compare the results of the different groups of HIV infected individuals as well as controls. Anemia and neutropenia were the most common hematological abnormalities observed in this study with highest prevalence of anemia found in the ART naive group. There was significant difference (pâ�?¤0.05) between groups in FSSA level. The highest levels of FSSA were observed in the HIV ART naive (0.65�?±0.5 mg/ml). The mean ESSA value for the study population was 0.54�?±0.35 mg/ml with no significant difference (pâ�?¤0.05) between groups. No significant difference (pâ�?¤0.05) was found between groups and also in gender and age. The findings in this study of higher mean sialidase activity and FSSA levels in the ART naive HIV group compared with other groups indicate that the virus and other opportunistic pathogens may be sialidase producers in vivo which cleave off sialic acids from erythrocytes surface, leading to high levels of FSSA, anemia and neutropenia seen in this group. The higher ESSA concentration found in the HIV stable group along with lowest FSSA concentration in the group suggests the presence of sialyltransferases.

Biography :

Email: khadeejahay@yahoo.com