Pharmaceutica Analytica Acta

Protein delivery system based on alginate microspheres produced using aerosolization technique

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Dewi Melani Hariyadi1, Allan Coombes2 and Esti Hendradi1

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Micro encapsulation of proteins in alginate microspheres was successfully produced using aerosolization technique. Insulin, ovalbumin and bovine serum albumin were used as model proteins with different molecular weight. The highest encapsulation efficiency and loadings was achieved by insulin-loaded alginate microspheres. An increase of protein molecular weight decreased encapsulation efficiency (from 50 to 32%) and protein loadings (from 51 to 3.3%). The lowest loading was BSA-loaded microspheres. Similar trend was found in case of microspheres size. Particle size reduced from 33 μm to 22.5 μm from microspheres encapsulated insulin to BSA. Dried morphology of microspheres revealed smoother spherical microspheres of BSA, ovalbumin and insulin respectively. Interestingly, in vitro release showed that alginate microspheres prevented release of insulin when incubated in HCl for 2h, followed by slower release of BSA (5%) and ovalbumin (40%). Complete release of 100% insulin, BSA and ovalbumin occurred after 8h, 14h and 15h incubation in PBS pH 7.4. The aerosolization technique demonstrated the potential of alginate microspheres for oral delivery of protein therapeutics.