Journal of Nanomedicine & Nanotechnology

Preparation and characterization of lipid nanoparticles containing photodynamic therapy drugs

22^nd International Conference and Expo on Nanoscience and Molecular Nanotechnology

November 06-08, 2017 | Frankfurt, Germany

Eshaan Soman, Mathias Viard, Bruce A. Shapiro and Anu Puri

RNA Structure and Design Section, RNA Biology Laboratory; National Institutes of Health, National Cancer Institute, Center for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Cancer nanomedicine is a promising area for improved drug delivery. Our objective is to develop light-sensitive liposomes (lipid bilayer nanoparticles) that will allow for drug release at the desired site in patients1,2. Photo-triggerable liposomes containing the photodynamic therapy (PDT) molecule HPPH and calcein (a water- soluble fluorescence molecule), have been demonstrated as suitable light-sensitive nanoparicles3 (U.S. Pat. App. 14/904,385)4. In this study, we have tested Chlorin e6 (Ce6), a PDT molecule that differs from HPPH in its structure but has a similar absorption spectrum. We examined the ability of Ce6 to package into the liposomes and its effect on the efficiency of calcein loading. The liposomes were tested for lasertriggered calcein release and Ce6 photodamage. Our data shows that (i) Calcein can be loaded into Ce6-containing liposomes with similar efficiency. (ii) Upon laser treatment, photodamage of Ce6 occurs, however, calcein release is less than that of HPPH-loaded liposomes. (iii) Ce6-loaded liposomes are more unstable at shorter intervals of time. Therefore, our data alludes to specific drug-lipid interaction requirements for optimal drug release from the liposomes. Our studies will aid in future clinical applications for localized delivery of multiple drugs.