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Performance of Labeo rohita larvae fed with raw seeds and various extractions of seeds of Achyranthes aspera and challenged with Aeromonas hydrophila
4th International Conference on Fisheries & Aquaculture
November 28-30, 2016 San Antonio, USA
Praveen Kumar Srivastava, Ruchi Singh and Deepak Chand Sharma
Dr. Shakuntala Misra National Rehabilitation University, India
Central Institute of Medicinal and Aromatic Plants, India
Posters & Accepted Abstracts: J Aquac Res Development
Abstract:
The present study evaluated the efficacy of dietary doses of various extractions of seeds of Achyranthes aspera powder on immunological parameters and disease resistance against the bacterial pathogen Aeromonas hydrophila infections in Indian major carp, Labeo rohita larvae. Larvae were fed with dry diet containing raw seeds of Achyranthes aspera, extractions of seeds of petroleum-ether extract (crude extract), and different percentage of petroleum ether-chloroform eluted fractions. Control diet was prepared without seed or extract of seed. Immunological (bactericidal, myeloperoxidase, NOS and lysozyme activity) parameters of fishes were examined at 41 days of feeding. Fishes were challenged with A. hydrophila 41 days post feeding and mortalities (%) were recorded over 7 days post-infection. The results demonstrate that fishes fed with seeds and various extractions of seeds of Achyranthes aspera showed enhanced bactericidal, myeloperoxidase, lysozyme activity, and total tissue protein level (p<0.05) compared with the control group. The survivability was higher in experimental diets than the control group. Dietary seeds of Achyranthes aspera showed significantly (P<0.05) higher protection against A. hydrophila infection than control. The results suggest that the seeds of Achyranthes aspera have a stimulatory effect on immunological parameters and increases disease resistance in Labeo rohita fingerlings against A. hydrophila infection.
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Email: praveen2ku28@gmail.com