Oral Health and Dental Management

Pancreatic lipase inhibitory activities of lactic acid bacteria isolated from raw camel milk

17^th World Congress on Oral Care and Probiotics

November 14-16, 2016 Orlando, USA

Priti Mudgil

United Arab Emirates University, UAE

Posters & Accepted Abstracts: Oral Health Dent Manag

Abstract:

Obesity, as an ever-growing epidemic, remains the top contributor to global burden of disease. As pancreatic lipase is the primary lipase that hydrolyzes dietary fat molecules in the human digestive tract and obesity is primarily a disorder of lipid metabolism, hence, selective inhibition pancreatic lipase could be targeted for its management. Lipstatin (Orlistat), a FDA approved pancreatic lipase inhibitor obtained from fermentation broth of Actinomycetes remains the most celebrated and successful anti-obesity drug till now. However, excessive inhibition of pancreatic lipase leads to certain discomforts like steatorrhea and isolated cases of organ toxicity. Till today, the ability of lactic acid bacteria (LAB) towards inhibition of pancreatic lipase remains to be unexplored. Hence, the present study was undertaken to explore the pancreatic lipase inhibitory potential of lactic acid bacteria isolated from camel milk. Streptococcus and Lactobacillus isolates were isolated from raw milk samples collected from local camel farms of Al Ain, Abu Dhabi, UAE. Further screening of lipase inhibitory activity using cell free extracts (CFE) was done and inhibitory activities were determined by comparing the release of p-Nitrophenol from P-Nitrophenyl palmitate through porcine pancreatic lipase type VI, in presence and absence of CFE using Orlistat as positive control while PBS, as negative control. Overall, lipase inhibitory profile of 97 LAB isolates and 11 reference strains was achieved. A wide variation in inhibitory activities was observed among isolates and reference cultures. The Streptococcus isolates (52) inhibition ranged from 3.0-99%. 11 isolates possessed potent inhibitory activities comparable or higher than Orlistat (83%; 2 mg/ mL). Among 45 isolates of lactobacilli negative inhibition was observed among 13 isolates, while for rest 32 isolates it ranged from 4.0-81%. Inhibition percentage in reference cultures ranged from 3.0-37%. L. acidophilus DSMZ 9126 showed strongest inhibition while L. gasseri 20243 showed lowest inhibition. These initial results definitively demonstrate that LAB isolated from raw camel milk have pancreatic lipase inhibitory activities and can reduce the hydrolysis of dietary fats in vivo and future use of probiotic bacteria in this field.

Biography :

Email: sazdulkarim@yahoo.com