Oral Health and Dental Management

Osteogenic differentiation of rabbit adipose-derived stem cells: A histomorphometry study

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Zahra Sarabadani

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Adipose tissue is an easily obtainable and robust source of adipose-derived stem cells (ADSC) which their role in regenerative process has been demonstrated in several studies. In this study ADSCs of intra scapular subcutaneous adipose tissue of rabbit had derived, then the possibility of their differentiation to the osteoblasts was evaluated by histomorphometrical analyses. Adipose tissue of intra scapular region of 12 male New Zealand rabbits was harvested during the direct surgery or liposuction. After stem cell preparation, they were culturing in a mixture of a kind of phosphate-based differentiation cocktail and growth media. Presence of osteocyte was evaluated via alizarin red staining and histomorphometry analyse. This study showed the differentiation of ADSCs to osteoblast in a phosphate-based matrix. However, more studies about the differentiation of ADSCs to variant cell lines and the essential matrix are necessary.