Oral Health and Dental Management

Microstrain of human root dentine after chelating agents’ application

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Abdillah Imron Nasution

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Objective: to determine the effect of EDTA and RcPrep to microstrain of human root dentine. Method: Fifteen extracted human premolars were sectioned at the cemento enamel junction using a diamond bur disc. Every group was then sectioned horizontally in the third cervical, third middle and third apical to have total of 45 specimens. Based on the test solutions used, specimens were divided randomly into three groups: (1) the EDTA group: 0.05 mL of 17% EDTA for 15 minute (n=15), (2) the RcPrep group, 0.05 mL of 15 % EDTA-urea peroxide-Carbowax for 15 minute (n=15), and (3) the control group, 1 mL of 0.9% saline for 15 minute (n=15). All specimens were prepared for the determination of microstrain of root dentine using X-Ray Diffraction (XRD) analysis. The microstrain of root dentine calculated using the equation ? = Br cos? / sin? then statistically analyzed using regression test. Result: Analysis of regression between control and treatment groups were significant (P<0.05). The microstrain was significantly greater with 17% EDTA when compared with 15% RcPrep. In addition, based on XRD result, all specimens showed same patterns as apatite groups. The pattern of RcPrep group showed better material than EDTA group. Conclusion: EDTA and RcPrep can contribute to improvements of microstrain of root dentine. In comparing these properties, the RcPrep showed lower effect than EDTA.