Agrotechnology

Lycopene and - carotene gene sequence polymorphism identified in contrasting tomato genotypes.

International Conference on Agricultural & Horticultural Sciences

September 14-15, 2012 Hyderabad International Convention Centre, India

Shilpa P, A.T.Sadashiva, K.V.Ravishankar, K. S. Shivashankar, Amrutha Sindhu B, Punith Kumar R, Kavitha P, Krithika T. K and M. George Christopher

Posters: Agrotechnol

Abstract:

Tomato (Solanum Lycopersicum) is one of the nutritive sources for Vitamin C and some major carotenoids like Lycopene and Vitamin A precursor β- carotene. Elucidation of the structure of these carotenoids has led to the study of genes and their ac - tion involved in biosynthesis of carotenoids. The genes that encode the enzymes of interest involved in biosynthesis of carotenoids were cloned and sequenced. Enzymes like CrtL (Lycopene β cyclase), Pds (Phytoene desaturase), crtR (β- carotene hydroxylase) from carotenoid pathway and Sus (Sucrose synthase) were used. Contrasting genotypes for lycopene and β- carotene were chosen and analysed by cloning the genes involved with the above enzymes. Sequence analysis showed Single Nucleotide Polymorphism at four positions in CrtL (Lycopene β cyclase) gene for IIHR 2101 with low lycopene content and β-carotene content compared to other lines such as IIHR 249-1-15-4, IIHR 2866 and Arka Vikas. Similarly Single Nucleotide Polymorphism at five positions for Sus (Sucrose synthase) in the sequence of A.Vikas when compared to other lines. Phytoene desaturase and β- carotene hy - droxylase also showed sequence polymorphism among the genotypes. Thus the difference in lycopene and β- carotene content in the genotypes may be due to the sequence polymorphism identified which may lead to amino acid changes and also the resulting end product.

Biography :

Shilpa.P is working as Senior Research Fellow at Molecular Biology Lab , IIHR at Bangalore.