Pharmaceutica Analytica Acta

Lipid complexed nanocrystals - A safe approach in development of stable nanoparticulates (in-vitro and in-vivo) for effective drug delivery

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Sajeev Kumar B

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Nanoparticulates (NPs) are developed with an aim to improve the solubility, targetability and bioavailability of a drug. Lipid Complexed Nanocrystals (LNCs) are novel and unique nanoparticulates offering high solubility and stability both in vitro and in vivo, hence making it more sustain to body fluids (electrolytes). In vitro instability (particle aggregation) of NPs may decrease its functional behavior in vivo leading to decreased bioavailability. Development of functional NCs requires modification of its surface properties in sequence to make them clinically more acceptable. The study aim to develop Glimepiride NCs using PEG 20000 by nanonization (precipitation) and stabilize them (both in vitro and in vivo) by complexation using Phospholipon 90 G (P 90G). Particle and solid state characterization studies were performed on NCs before and after complexation using photon correlation spectroscopy (PCS) and X-ray diffraction spectrometry (PXRD), differential scanning calorimetry (DSC), scanning electron spectroscopy (SEM) and fourier transform infrared spectroscopy (FTIR). In vivo drug targeting efficacy of LNCs was studied on pancreas of Male Wistar rats using HPLC. The particle and solid state characterization results show improved stability (decreased aggregation) with no change in drug properties after complexation. In vivo results on optimized LNCs show similar drug concentration in pancreas of rat as that of pure drug. AUC was significantly higher after 1 h signifying better in vivo stability. It can be concluded that in vitro and in vivo stability of NCs could be achieved by a complexation using P90 G. The possible outcome of these studies could result in development and delivery of stable and safe nanoproducts in the treatment of diabetes.