Journal of Aquaculture Research & Development

Isolation of potential probiotic Paenibacillus ehimensis NPUST1 with bacteriocin activity and its effect on growth performance and immunity against Streptococcus iniae in Nile tilapia (Oreochromis niloticus)

11^th Global Summit on Aquaculture & Fisheries

May 24-25, 2018 Osaka, Japan

Shao-Yang Hu and Sai-Wei Chen

National Pingtung University of Science and Technology, Taiwan

Posters & Accepted Abstracts: J Aquac Res Development

Abstract:

Probiotics used as immunostimulants is an emerging strategy in aquaculture for prophylactic biocontrol. In the present study, a probiotic Paenibacillus ehimensis NPUST1 producing antimicrobial substance was isolated from tilapia culture pond and characterized by biochemical analysis and 16S rDNA sequencing. The physiochemical properties of crude extracted antimicrobial substance revealed low pH and highly thermal tolerance. The antimicrobial activity of crude extracted antimicrobial substance exhibited broad spectrum against diverse aquatic pathogens, food spoilage, clinical pathogens and plant pathogen. The effect of dietary supplementation with P. ehimensis NPUST1 on the growth of Nile tilapia (Oreochromis niloticus) and immunity against Streptococcus iniae was evaluated. The results showed that Weight Gain (WG), Feed Conversion Ratio (FCR) and Feed Efficiency (FE) of Nile tilapia fed P. ehimensis NPUST1 for 2 months were significantly increased compared with those of the fish fed control diet. Nile tilapia fed P. ehimensis NPUST1 exhibited a higher survival rate than fish fed control diet following challenge by Streptococcus iniae. The immune parameters revealed that phagocytic activity, respiratory burst and Superoxide Dismutase (SOD) of head kidney leukocytes as well as the serum lysozyme activity of P. ehimensis NPUST1-fed Nile tilapia were significantly higher than fish fed the control diet for 2 months. These results indicate that dietary supplementation with P. ehimensis NPUST1 improved growth performance and enhanced immunity and disease resistance in Nile tilapia.

Biography :

Shao-Yang Hu has earned his PhD degrees in Microbiology and Biochemistry from National Taiwan University and Postdoctoral studies in Institute of Cellular and Organismic Biology (ICOB), Academia Sinica. He is the Chief of Interdisciplinary Feature Development Center of National Pingtung University of Science and Technology (NPUST). He has published more than 20 papers in reputed journals.
Email:syhu@mail.npust.edu.tw