Biochemistry & Analytical Biochemistry

Isolation and expression analysis of a cDNA encoding for phosphoenolpyruvate carboxylase in thermogenic skunk cabbage, Symplocarpus renifolius

International Conference on Biochemistry

October 10-12, 2016 Kuala Lumpur, Malaysia

Md Abu Sayed, Yui Umekawa and Kikukatsu Ito

Iwate University, Japan

Posters & Accepted Abstracts: Biochem Anal Biochem

Abstract:

Skunk cabbage, Symplocarpus renifolius, is known to produce enough heat to melt the snow in early spring. We have isolated a cDNA that encodes a phosphoenolpyruvate carboxylase, termed as SrPEPC from thermogenic florets of skunk cabbage. SrPEPC is a 110.5 kDa protein that contains conserved amino acid regions such as phosphorylation and catalytic domains. Expression of SrPEPC mRNA was highly abundant in the petal and pistil of thermogenic florets. Interestingly, the expression was very low in nonthermogenic tissues including spathe and leaf. Our data suggest that SrPEPC plays a role for tissue-specific heat production in the skunk cabbage.

Biography :

Md Abu Sayed is currently pursuing his PhD from United Graduate School of Agriculture, Iwate University, Japan. He has been serving as an Assistant Professor in the Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur, Bangladesh since 16 May, 2012.

Email: u0414002@iwate-u.ac.jp