Biochemistry & Analytical Biochemistry

Inorganic phosphorus and potassium are putative indicators of delayed separation of whole blood

International Conference on Clinical Chemistry & Laboratory Medicine

October 17-18, 2016 Chicago, USA

So Youn Shin

Korea Institute of Tuberculosis, South Korea

Posters & Accepted Abstracts: Biochem Anal Biochem

Abstract:

The delayed separation of whole blood can influence the concentrations of circulating blood components. We aimed to determine whether clinical-biochemistry analytes can be used to assess the delayed separation of whole blood in bio-bank. We investigated the plasma and serum concentrations of five clinical-biochemistry analytes and free hemoglobin when the centrifugation of whole blood stored at 4 °C or room temperature was delayed for 4, 6, 24 hours, or 48 hours, and compared the values with those of matched samples that had been centrifuged within 2 hours after whole-blood collection. The inorganic phosphorus (IP) levels in the plasma and serum samples were elevated �?�1.5-fold when whole-blood centrifugation was delayed at room temperature for 48 hours. Furthermore, the IP levels in the plasma samples showed excellent assessment accuracy with AUC>0.9 after a 48-hour delay in whole-blood separation, and high sensitivity (100%) and specificity (95%) at an optimal cutoff point. The IP levels in the serum samples also exhibited good assessment accuracy with AUC>0.8, and high sensitivity (81%) and specificity (100%). The potassium (K+) levels were elevated 1.4- fold in the serum samples following a 48-hour delay in whole-blood separation. The K+ levels showed excellent assessment accuracy (AUC>0.9) following a 48-hour delay in whole-blood separation, and high sensitivity (95%) and specificity (91%) at an optimal cutoff point. Our study showed that the IP and K+ levels in the plasma or serum samples could be considered as putative indicators to determine whether whole-blood separation had been delayed for extended periods.

Biography :

Email: leukoso@hanmail.net