Oral Health and Dental Management

In vivo CS filled HA/TCP lattice-scaffold with PDGF sustained release

International Conference and Exhibition on Dentistry

March 18-20, 2015 Dubai, UAE

Murriky, Cornelia, Elizabith, Alessandro, Steven Jim and Jack

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Objective: To study the effect of PDGF (platelet derived growth factor) in rate and quality of bone development when added to HA/TCP (hydroxyapatite/tricalciumphosphate) lattice bone scaffolds, temporarily filled with calcium sulfate(CS). Bone growth into scaffolds depends in part on vasculogenesis which is encouraged by sustained release of PDGF. CS as a drug carrier shows sustained release rather than a burst release. Methods: 3D-printed HA/TCP lattice scaffolds (11mm diameter?3mm disk struts 250μm diameter, quadranted pores 250?250, 250?400, 400?250 and 400?400μm) were temporarily filled with CS (control) and 1.5μgPDGF/0.2gm CS. Soft tissue ingrowth prevented by solid cap at the top of the scaffold. They were implanted in bilateral critical sized trephine defects in rabbit parietal bone for 8 and 16 weeks. Light and scanning electron microscopy, microCT and histology were used to analyze tissue response. Bone penetration was analyzed using microCT scan of the upper complete layer and the lower complete layer. Result: MicroCT of 8 weeks specimens showed bone penetration of 62%, compared to control group 58% penetration. Small and medium pored showed more bone deposition than large pores. Bone growth conducts along the struts and start from the edge of the specimen toward the center. Samples with PDGF have more remodeling than the controlled. In specimen with PDGF there is four samples reached the center in upper and lower complete layer, small and medium pores compared to tow samples without PDGF. Conclusion: Results suggest that HA/TCP scaffolds remodel extensively by osteoclastic resorption, and that this is increased by PDGF. Difference in bone penetration between the 2 groups is small at 8 weeks. The pattern and extent of bone formation is influenced by scaffold configuration.