Journal of Nanomedicine & Nanotechnology

In situ solid lipid nanoparticles of lopinavir: A simple technology to address complex biology

5^th International Conference on Nanotek & Expo

November 16-18, 2015 San Antonio, USA

Maithania Heena V, Shinde Umesh K and Devarajan Padma V

Institute of Chemical Technology, India

Posters-Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Nano drug delivery technology offers the advantage of enhanced efficacy of drug molecules by altering its pharmacokinetic and pharmacodynamic properties. Although the highly active antiretroviral therapy (HAART) has reduced morbidity and mortality rate due to HIV, complete cure is not achieved due to insufficient drug concentration in the cellular and anatomical reservoirs of the body where the HIV harbors. In the present investigation, we present in situ SLN of Lopinavir based on simple technology, for simultaneous targeting of the reticulo-endothelial system (RES) reservoirs and also remote HIV reservoir sites in vivo. The in situ SLN concept is based on simple mixing of two components, component-A comprising Lopinavir, lipid and stabilizers and component B comprising stabilizers in an aqueous phase. Mixing components A and B results in the generation of nanoparticles in situ, with a high entrapment efficiency of Lopinavir >85% and an average size in the size range 200 nm to >1000 nm. Lipids evaluated consist of fatty acids, alcohol and stearates. The nature of lipid and vehicle used influenced both size and efficiency. An in situ SLN system with an average size of 300 nm and entrapment efficiency >85% with good reproducibility and stability was optimized. Pharmacokinetic data revealed increased t1/2 and volume of distribution (Vd) values. Bio-distribution study of the in situ SLN revealed high Lopinavir concentration in the RES reservoir sites of HIV such as liver, lungs, spleen, kidney and remote sites of brain and lymph node. In situ SLN provides simple technology to address a complex biological need, for effective anti-HIV therapy.

Biography :

Email: heena_maithania2@yahoo.com