Journal of Aquaculture Research & Development

Identification and characterization of an 18.4 kDa antimicrobial truncation from shrimp Litopenaeus vannamei hemocyanin upon Vibrio parahaemolyticus infection

5^th Euro Global Summit on Aquaculture & Fisheries

March 30-31, 2017 Madrid, Spain

Yue Ling Zhang, Ying Wen, Shixiong Zhan, He Huang, Mingqi Zhong and Fan Wang

Shantou University, China

Posters & Accepted Abstracts: J Aquac Res Development

Abstract:

Hemocyanin (HMC) is a multifunctional protein which plays many essential roles in invertebrate organisms. Recently more and more immune-related functions have been discovered on this protein. Here, the shrimp was infected with Vibrio parahaemolyticus and the shrimp sera were analyzed by two-dimensional gel electrophoresis. Totally 15 spots were identified as significantly up-regulated spots and further analyzed by MALDI-TOF/TOF mass spectrometry. Four of them were identified as HMC derived truncations (HMCS1, HMCS3, HMCS4 and HMCS5). The HMCS4 primary sequence was further determined via Edman N terminal sequencing, MALDI-TOF MS and amino acid sequence alignment. The result indicated that the HMCS4 was a 165aa fragment from shrimp HMC small subunit C-terminal. The HMCS4 immunological activities were further analyzed by agglutination experiment and antibacterial assay in vitro. The results showed that the recombinant HMCS4 had strong agglutination and antibacterial activities against pathogenic bacteria at the optimum bacteriostasis concentration. In addition, the HMCS4 immunological activities were explored via mortality assay in vivo. The shrimp was challenged with V. parahaemolyticus and HMCS4 V. parahaemolyticus mixture separately. The shrimp mortality rate significantly decreased at 96 h post-infection with HMCS4 injection. Our data showed that shrimp HMC truncation generation upon infection was an effective immune response against invaded pathogens. Moreover, these findings may have some potential applications in shrimp industry.

Biography :

Yue-Ling Zhang is a PI in Guangdong Provincial Key Laboratory of Marine Biotechnology, School of Sciences, Shantou University. He received his PhD from Xiamen University, China and studied at Uppsala University, Sweden as a visiting scholar. He was a Professor and Group Leader of Department of Biology, Shantou University, China from 11/2011 to 03/2013, 05/2014 to 11/2016, and then moved to Marine Biology Institute, Shantou University, China as a Professor and Group Leader since 11/2016. He is the Director of Guangdong Province Society for Biochemistry and Molecular Biology, a Standing Director of Guangdong Province Society for Biochemistry and Molecular Biology, a Director of Guangdong Province Society for Zoology, and a Director of Guangdong Province Society for Aquaculture. His main research is focusing on shrimp immunology, especially the immune mechanisms of crustacean hemocyanin. So far, he has been the recipient of about 20 foundations as Principal Investigator. In peer-reviewed publications, his group has published 46 research papers in J Proteome Res, Fish Shellfish Immunol, FEBS Lett, Dev Comp Immunol, Immunol Lett, PloS ONE , Mol Immunol, J Shellfish Res and so on.

Email: zhangyl@stu.edu.cn