Biology and Medicine

Growth and biochemical composition of molecular identified marine microalga Nannochloropsis oceanica, cultured under nutrient limitation on reduced cost media for aquaculture and biodiesel production

2^nd World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry

September 22-24, 2016 Phoenix, USA

Abd El-Wahab Kamil and M Ashour

National Institute of Oceanography and Fisheries, Egypt

Posters & Accepted Abstracts: Biol Med (Aligarh)

Abstract:

Microalgae culture media should be economical, allow for high growth, satisfy the needs of microalgal cells and easy to prepare. In this study, we evaluate the effect of different medium formula prepared from commercial agricultural fertilizers (CAGF), comparing to F/2 Guillard medium as control medium on growth (specific growth rate, μ; cell density, CD; and dry weight, DW) and biochemical composition (lipid, protein, and carbohydrate) of Nannochloropsis oceanica, identified by 18S RNA. Comparing to N/P ratio of F/2 Guillard medium, six treatments of N/P ratios (1.6, 3.2, 4.8, 9.6, 10.3 and 20.7) were prepared from ammonium sulfate (NH) or nitric acid (NU) as a nitrogen source with phosphoric acid (P) as phosphorus source. The results investigated that some CAGF media achieved significant (P�?�0.05) growth and biochemical composition higher than F/2, depending on N/P ratios and atomic sources concentrations. The highest CD (9.325±0.855�?107 cell/ml), DW (0.9060±0.0274 g/l) and μ (0.6500±0.223 Division/day) was achieved by treatment NU100+P100, while the lowest CD (4.400±1.710�?107 cell/ml) and DW (0.7484±0.0547 g/l) were achieved by treatment NU50+P300. Comparing to F/2 control lipids (30.70%), protein (14.47%) and carbohydrates (8.31%), the highest significant total lipid (48.62±5.08%) was achieved by NH100+P300, while the lowest (18.40±1.39%) were observed by NU50+P300. Furthermore, NU100+P300 and NH50+P100 were achieved the highest protein (28.46±2.39% and 28.40±2.47%), respectively, while the lowest (10.27±2.39% and 10.65±0.72%) were achieved by NH50+P50 and NH100+P100, respectively. The highest carbohydrate (38.50±1.21%) was achieved by NU50+P50, while the lowest was achieved by F/2 Control (8.312±4.47%), followed by NU100+P50 (8.64±2.73%), NH50+P300 (9.62±2.42%) and NU50+P300 (9.84±1.15%), respectively. From the previous data we can conclude that the different nitrogen sources may affect the growth and biochemical composition of N. oceanica, depending on the atom concentration and N/P ratio. Finally, the use of CAGF constitutes a viable alternative to reduce production costs and it also can substitute the F/2 media which is commonly used for culture of N. oceanica in the fields of aquaculture and biodiesel production.

Biography :

Email: abdelwahab.kamil@yahoo.com