Journal of Aquaculture Research & Development

Genotyping crayfish plague fungus Aphanomyces astaci by using RAPD from Turkey

7^th International Conference on Aquaculture & Fisheries

October 19-21, 2017 | Rome, Italy

Ifakat Tulay Cagatay, Suleyman Akhan, Selcuk Berber, Busra Arli, Yigit Tastan and Tuba Catli

Akdeniz University, Turkey

Posters & Accepted Abstracts: J Aquac Res Development

Abstract:

Aphanomyces astaci is an agent of the crayfish plague which causes mass mortality and economic losses for fishery. While isolating methods have been improved, low-level of infection are still virtually difficult by culture methods. Molecular methods offer faster and specific diagnosis and genotyping. Two different primer sets (OB1 and OB3) were chosen for a comparative analysis of different A. astaci isolates by using RAPD-PCR. In our preliminary results showed that different geographic isolates of crayfish from Manyas and Bafra Lakes population seem to carry different strains of A. astaci. RAPD data suggested that two Turkish A. astaci isolates are highly similar to genotype B which was our positive control. Learning on the diversity of this fungus could help to identification of different genotypes in crayfish population in Turkey and will also guide tracing the sources of this infection in worldwide. Therefore, attempt to characterize new A. astaci genotype will continue for further works.

Biography :

Ifakat Tulay Cagatay has completed her Master degree in Microbiology at Hacettepe University, Ankara, Turkey, in 1997 and PhD in Molecular Microbiology at Lincoln University, New Zealand, 2005. She has been working at Akdeniz University as a Researcher and Lecturer. Recently, she and her group are working on bacterial fish diseases projects and also lecturing microbiology, genetics, molecular biology and marine microbiology.