Oral Health and Dental Management

Formulation and characterization of antimicrobial herbal mouthwash from some herbal extracts for treatment of periodontal diseases

3^rd Euro Congress and Expo on Dental & Oral Health

June 16-18, 2015 Alicante, Spain

R Yadav1, A Asthana2 and S Yadav1

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Purpose: The purpose of the present work was to formulate herbal mouthwash with antimicrobial property which is expected tocure/protect from various periodontal diseases such as periodontitis, gingivitis, and pyorrhea. Methods: Plant materials, extracted and standardized. Screening of antimicrobial activity was carried out with the help of disk diffusionmethod. Minimum inhibitory concentration assay was performed by agar dilution method recommended by the NationalCommittee for Clinical Laboratory Standards. Mouthwash was formulated by Dried Extracts of Cuminumcyminum, Beautea monosperma, and Cordiaobliqua. and evaluated on various parameters and Standardization of the formulation wasperformed. Organoleptic characterization was performed at every stage of the development of the formulation. formulationwas standardized by taking HiOra as standard marketed formulation.Total phenolic and flavonoid contents were estimated by folin-Ciocalteu and aluminium chloride method, and stability studies were performed.40oC and RH 75% � 5% for 90 days)to assess the effect of temperature and humidity on concentration of phenolic and flavonoid contents. Results: Results are encouraging, as all other antibiotics were inactive against these strains. extracts from Cordiaobliqua, Beauteamonosperma and Cuminumcyminum possess significant antimicrobial activity at very low oncentration (15?g/ disc,20?g/disc and 15?g/disc).Chemical tests shown presence of flavonoids, phenolics in the extracts, might be responsible for the activity. Evaluation of the formulation showed all the values in limit.And shown ideal values forall parameters. Acceleratedstability studies are indicative of a slight reduction in flavonoids and phenolic contents with time on long time storage. Fromthe results it can be concluded that flavonoid and phenolic contents are reducing with time by the effect of the temperature and moisture. When measured degradation under ambient conditions, degradation wassignificantly lower than in acceleratedstability study. Conclusion: The results of the study support traditional application of the plants and suggest, plant extracts possess compounds withantimicrobial properties that can be used as potential antimicrobial agents Developed formulation will cure/protect fromvarious periodontal diseases. Further development and evaluation of mouthwash including the isolated compounds on commercial scale and their clinical and toxicological studies are the future challenges