Journal of Nanomedicine & Nanotechnology

Exosome nanovesicles for the homing delivery of therapeutics into autologous cancer cells

Joint Event on 23^rd International Conference on Nanomaterials science & Nanoengineering & Technology & International Conference and Exhibition on Pharmaceutical Nanotechnology and Nanomedicine

April 18-19, 2018 | Las Vegas, USA

Shuhua Bai, Tianzhi Yang, Judy Nguyen, Denise Lyakhovich and Erica Meehan

Husson University, United States

Scientific Tracks Abstracts: J Nanomed Nanotechnol

Abstract:

Extracellular vesicles (EVs) are naturally occurring membrane particles that mediate intercellular communication by delivering molecular information between cells. In this study, we propose to package therapeutics agents in endogenous nanovesicles, known as exosomes, derived from cancer cells. It is expected that autologous cancer cell-derived exosomes can be taken by cancer cells themselves via homing selectivity and bring the entire payload to the autologous tumor cells. Exosomes were isolated from human adenocarcinoma A549 and brain endothelial bEND.3 cell cultures using centrifugation. They presented nanosized vesicles measured by a nanosizing system. Fluorescent rhodamine 123 and anticancer drugs including paclitaxel and doxorubicin were incorporated into exosome nanoparticles by diffusion. Designed siRNA to inhibit Intercellular Adhesion Molecule-1 (ICAM-1) was loaded into exosome with the assistance of transfection reagent. Fluorescence intensity in the cells treated with autologous cell-derived exosome delivered markers was significantly increased. Exosome-delivered anticancer drugs significantly decreased cell viability in autologous cells compared to other exosomes. Exosome-delivered siRNAs significantly enhanced the knockdown efficacy compared to siRNA alone, dependent on exosome original sources and loading methods. ICAM-1 siRNA formulated in autologous lung cell-derived exosomes with the assistance of transfection agent showed the best inhibitory effect on the ICAM-1 expression compared to other treatments. Cancer cell-derived exosomes could be used as effective carriers, bringing therapeutic agents into the cells and increasing efficacy to their parental cells. The use of cancer cell-derived exosomes will be further investigated for individualized and targeted lung cancer therapy.

Biography :

Shuhua Bai has obtained his PhD from the School of Pharmacy, Texas Tech University Health Sciences Center. He is an Associate Professor in the School of Pharmacy, Husson University. He has had more than 16-years of research experience on the Nanoparticle-based drug delivery and development. He has published more than 30 research papers in reputed journals. He recently received the 2017 Pharmaceutical Research Meritorious Manuscript Award, presented by the American Association of Pharmaceutical Scientists (AAPS).
Email:bais@husson.edu