Biology and Medicine

Evidence for plasmid-conferring salt in root associated bacteria from halophytes (Haloxylon recurvum and Atriplex amnicola) and non-rhizospheric soil samples

2^nd World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry

September 22-24, 2016 Phoenix, USA

Salma Mukhtar, Muhammad S Mirza, Samina Mehnaz and Kauser A Malik

Forman Christian College, Pakistan
National Institute for Biotechnology and Genetic Engineering, Pakistan

Posters & Accepted Abstracts: Biol Med (Aligarh)

Abstract:

Main purpose of this research was to investigate the evidence for plasmid conferring salt tolerance in rhizospheric bacteria isolated from Haloxylon recurvum and Atriplex amnicola. 22 strains were selected after screening of salt tolerance. Selected strains were characterized morphologically, biochemically and identified by PCR amplification of specific 16S rRNA gene sequences. Plasmid curing of isolates was done by heat shock method to study the effect of plasmid conferring salt tolerance. These plasmids were isolated and transformed into E. coli and growth response of original strains and transformed E. coli was compared at 1.5-4M NaCl concentration. Almost all strains showed optimum growth at 1-3.5M NaCl. These strains were related to Bacillus spp., Pseudomonas sp., Streptomyces sp., Halomonas spp., Citricoccus sp., Halobacterium sp., and Kocuria polaris. Cured isolates showed no growth in halophilic medium but grew well on LB medium. Biochemical and morphological characterization of cured strains was done and compared with the original strains. Various biochemical changes were observed after plasmid curing. Certain traits other than salt tolerance were plasmid linked and on curing, either the loss of the complete plasmid or a part of the plasmid resulted in the loss of specific characteristics. Mostly transformed E. coli were able to grow up to 3M NaCl concentrations. Different salt tolerant will be characterized and can be used to develop transgenic plants having salt tolerance.

Biography :

Email: salmamukhtar85@gmail.com