Oral Health and Dental Management

Evaluation of antimicrobial effect of ozonated water, chlorehexidine, amoxicillin, metronidazole and amoxicillin-metronidazole on Porphyromonas gingivalis: An in-vitro study

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Zahra Sarabadani

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Background: The use of ozone in dentistry has been proposed due to its antimicrobial, disinfectant, biocompatibility and healing properties. Gingival and periodontal diseases play a key role in dentistry. The development of periodontal diseases has been thought to be associated with the presence of several anaerobic bacteria. These species mainly include Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, in the subgingival environment. The primary objective of the present study was to evaluate the antimicrobial effect of ozonated water, chlorehexidine, amoxicillin, metronidazole and amoxicillin-metronidazole on Porphyromonas gingivalis. Materials & Methods: Double-blind in-vitro study was performed to investigate the effect of different concentrations of ozonated water, chlorehexidine, amoxicillin, metronidazole infusion, metronidazole suspension and amoxicillin-metronidazole suspension on the Porphyromonas gingivalis (n=840) growth. The minimum inhibitory and bactericidal concentrations of these materials were measured and data were statistically analyzed by two-way ANOVA and LSD methods. Results: Based on the results ozonated water, chlorehexidine and amoxicillin-metronidazole suspension have significantly higher antimicrobial effect than metronidazole suspension, metronidazole infusion and amoxicillin on Porphyromonas gingivalis (p<0.05) Conclusion: Due to the strong antimicrobial effect of ozonated water on Porphyromonas gingivalis, it can be use as an adjunct antiseptic in periodontitis therapy. Although more clinical and in-vitro studies are in demand to standardize indications and treatment protocols of this promising medical agent in dentistry.