Pharmaceutica Analytica Acta

Effects of the Vagus Nerve Changes in High Fat Diet Induced Obesity: One of Links for Diabetic Gastroparesis (A Histological and Morphometrical Study)

23^rd Global summit on Analytical and Bioanalytical Techniques

September 12-13, 2022 | Webinar

Altunkaynak BZ, Baycu C

Dept of Histology and Embryology, Medical Faculty, İstanbul Okan University, İstanbul-Turkey

Scientific Tracks Abstracts: Pharm Anal Acta

Abstract:

Obesity is a serious disease caused by excessive accumulation of fat in the body. Diseases caused by overweight, such as type 2 diabetes and high blood pressure, increase the risk of death. The use of applications performed over the vagus nerve in the treatment of obesity is becoming more and more common on the world. In this study, the effect of vagal inhibition on gastric morphology in a high-fat diet-induced obesity model was investigated. In this study, adult female rats were fed HFD or standard diet (controls) for 8 weeks. At the end of this nutritional process, all animals in the sham and VNI groups were anesthetized; also, sham and crushing operations of vagus nerve were performed on the groups, respectively. Following the recovery period, stomachs were removed from the rats. Volume of stomachs was detected with water injection method. For this step, water was injected into stomach until water was seen at the lower esophagus. Then stomachs were prepared for light microscopical examination and it was evaluated histologically. In the sections of high fat diet fed rats, not the lamina propria but submucosa is infiltrated by white blood cells and also many macrophages were seen. In sections of animals with crushed vagus nerve; volume of the stomach was decreased and histological appearance of the stomach was normal. The study indicated that factors that control stomach volume and structure depend on body size and metabolism. Also vagus nerve can be a potential targets in fighting obesity and related type 2 diabetes

Biography :

B. Zuhal Altunkaynak is Professor in histology and embryology. He has expertise in toxicological studies in the animal models. Also he studies on new pathways for improving healthcare. He uses light and electron microscopy, stereology and also histochemical and molecular techniques for her studies.