Pharmaceutica Analytica Acta

Discovery and purification of novel antimicrobial protein: An alternative bio-therapeutic to conquer multidrug resistant organisms prevalent in the community

5^th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems

March 16-18, 2015 Crowne Plaza, Dubai, UAE

Sidra Pervez, Asma Ansari, Shah Ali UlQader

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Nowadays, increased antibiotic resistance of multi-drug resistant organisms has become a hot topic in healthcare sectors. Most of the effective drugs are now turn out to be nearly useless against these organisms. This public health dilemma is increasing globally due to over-prescription of medicines by doctors as well as increase use and misuse of these drugs by patients. Most of the bacteria causing hospital acquired infections are now resistant to at least one or more than one commonly used drugs. Threat posed to public health by multidrug resistant organisms can be resolved by the discovery of new antimicrobial compounds. This concern is becoming a challenge for researchers to investigate a natural antimicrobial compounds. Among these antimicrobial compounds, bacteriocins are highly specific antagonists having broad inhibitory spectrum. Bacteriocin (BAC-IB17) produced by Bacillus subtilisKIBGE-IB17 was found to be effective against various pathogenic strains. A low molecular weight BAC-IB17 was purified to homogeneity through gel permeation chromatography with a final specific activity of 186 AU mg-1. This unique bacteriocin is found to be highly thermostable and pH stable in nature. BAC-IB17 also showed its stability against various heavy metals, organic solvents, surfactants and proteolytic enzymes. N-terminal sequencing and aminoacid analysis was performed for complete characterization and classification ofbacteriocin. These findings suggests its applicability for the development of effective bio-therapeutics. BAC-IB17 exhibits a good potential for clinical and industrial applications and it can be a plausible candidate among other newly discovered drugs.