Apurva R. Shah and Rinti Banerjee
Scientific Tracks Abstracts: PAA
ackground:Pulmonary surfactant (PS) plays a pivotal role in normal breathing process and prevention of alveolar collapse by dynamically modulating surface tension (from ~25 to near 0 mN/m surface tension) at air-liquid interface. PS composition is altered/destroyed due to high oxidative stress and fl uid fatty acids produced in infl amed lungs thereby rendering PS dysfunctional. In the present study, surface active Dipalmitoyl phosphatidylcholine (DPPC) liposomal system with active therapeutic adjuvant D-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS) has been formulated as a protective aerosol in oxidative stress. Method: Tensiometric analysis of phospholipid-TPGS monolayer fi lms was done in Langmuir-Blodgett trough. Liposomal formulations were prepared using thin fi lm hydration method. Particle size and shape characterization of potential liposomal formulations was done using dynamic light scattering and electron microscopy. Lipid peroxidation of soybean lecithin in presence and absence TPGS was done by thiobarbituric acid assay (TBARS) method. In-vitro biocompatibility of developed liposomal formulation was evaluated in A549 cell line. Developed liposomal system was also evaluated for airway patency maintenance using capillary surfactometer. Result: DPPC:TPGS 1:0.25 to 1:1 w/w Langmuir fi lm attained near zero surface tension like natural PS. Adsorption isotherm studies showed additing incremental amount of TPGS to DPPC liposomes aid lowering surface tension reached in 1 second from 46.41 ? 0.57 mN/m to 40.41 ? 0.55 mN/m. Liposome particle size were in the range of 200-350 nm. Incremental addition of TPGS reduced lipid peroxidation of soybean lecithin liposomes lowering TBARS value by 29-52 %. DPPC:TPGS liposomal formulation were found to be biocompatible and maintained capillary (airway) > 95 % patent. Conclusion: An artifi cial PS with improved surface activity and antioxidant capability has been developed. Th e liposomal system is biocompatible and maintains capillary (airway) patency making it a promising adjuvant for therapy in respiratory infl ammation.
Apurva R. Shah is presently a Ph.D student guided by Prof. Rinti Banerjee at Indian Institute of Technology Bombay, India. His research area involves design and development of drug and polymer added lipid based novel drug delivery system for respiratory ailments. He has couple of publications under his name related to his research area.