Oral Health and Dental Management

Comparison of antimicrobial efficacy of triple antibiotic paste, calcium hydroxide, nano calcium hydroxide, mixture of nano calcium hydroxide with triple antibiotic paste and metapexagainst entrococcusfaecalis: An in vit

International Conference and Exhibition on Dentistry

March 18-20, 2015 Dubai, UAE

NehaAgrawal

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

Introduction:The purpose of this study was to determine the in vitro antimicrobial efficacy against Entrococcus (E.) faecalis of triple antibiotic paste compared with calcium Hydroxide,nano calcium hydroxide mixture of nano calcium hydroxide with triple antibiotic paste and metapex. Materials and Methods: An agar well diffusion assay and MIC method were used to determine the efficacy of the experimental medicaments in removing E. faecalis. Medicaments were divided into 5 groups; triple antibiotic paste, calcium Hydroxide, nano calcium hydroxide,metapex and mixture of nano calcium hydroxide with triple antibiotic paste. The diameters of the growth inhibition zones for each group were recorded and compared. The minimum inhibitory concentrations (MIC) of tested medicaments that are required to kill E. faecalis were also determined. The differences between groups were analysed by kruskalwallis and mannwhitney u test Results: The largest inhibition zones were observed for the triple antibiotic paste and mixture of nano calcium hydroxide with triple antibiotic paste, and the smallest for Ca(OH)2. Concentration increases produced greater antibacterial effects in all groups. The MIC determination method showed similar results. Conclusion: The results suggest that the triple antibiotic paste and mixture of nano calcium hydroxide with triple antibiotic paste would be the preferred medicament against Entrococcus Faecalis. The nano calcium hydroxide showed better results than calcium hydroxide and metapex.