Pharmaceutica Analytica Acta

Characterizing interaction of chromatographic beads with Hansenula polymorpha by force spectroscopy and XDLVO

Annual Pharmaceutical Biotechnology Congress

May 16-17, 2018 Singapore

Cristina Chiutu, Vikas Yelemane, Alexander Neveshkin, Marcelo Fernandez-Lahore and Jurgen Fritz

Jacobs University Bremen, Germany

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Project aims to quantify the interactions that occur at nanoscale between biomass and chromatographic beads in real-life expanded bed adsorption. Force spectroscopy can be probed with AFM between single chromatographic beads and a cellular layer of Hansenula polymorpha or a thin film of mannan sugar. The work included in this presentation focused on probing interaction of Source S (negative) and Source Q (positive) beads with Hansenula polymorpha in phosphate buffer of different NaCl concentration. The results of force spectroscopy are compared against XDLVO calculations obtained from contact angle and zeta potential measurements. The beads (Fastline or Source) were tested with AFM on a clean silicon surface before performing the measurements on cells. The beads showed good reproducibility and stability regarding electrostatic repulsion or attraction. Characterization of beads at nanoscale as well as a detailed analysis of their behavior when interacting with biomass may lead to higher efficiency and performance in downstream processing biotechnology. cristina.chiutu@kaust.edu.sa cristinachiutu@yahoo.com