Biology and Medicine

Characterization of indigenous Bacillus thuringiensis strains based on their cry genes and Cry protein profile

2^nd World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry

September 22-24, 2016 Phoenix, USA

Shakila Sultana, Asaduzzaman Shishir, Shakila Nargis Khan and Md Mozammel Hoq

University of Dhaka, Bangladesh

Posters & Accepted Abstracts: Biol Med (Aligarh)

Abstract:

Bacillus thuringiensis (Bt) is widely used biopesticide. The aim of this study was to characterize indigenous Bt strains by their Cry protein profiling and to establish a correlation between the cry genes and their Cry protein profile. Through analyzing 21 strains by SDS-PAGE at 20, 40 and 72 hours, prominent protein bands were assigned presumptive Cry protein classes based on their molecular weight. From the whole cell protein profile at different time, it was observed that the molecular weight of the Cry proteins obtained from the indigenous Bt strains were in the range from 17 to 165 kDa. Cry1A class was the most prevalent among the strains. Cry2, Cry3, Cry6, Cry9 and cytotoxin class of endotoxins were also frequently found. Bt strains harboring potential Cry toxin classes (Cry1, Cry2, Cry3 and Cry9) were subject to PCR for the confirmation of the presence of respective cry genes. Twelve of the isolates showed the presence of Cry1A toxin and cry1A gene. Four isolates were found to contain Cry3 toxin and cry3 gene, four isolates showed the presence of cry9 gene and only one isolate showed the presence of the cry2 gene only. Thus correlation between the cry gene and Cry protein class was found. From the chronological protein profiles of the isolates it was presumed that protease activity might be present that was degrading the Cry proteins gradually. Ten of the isolates showed increasing protease activity while their protein concentration decreased with time. Finally RAPD genotyping of the isolates showed close relationships among the strains that contained cry1A and cry9 genes.

Biography :

Email: sultanashakila130@gmail.com