Biodiversity of naturally transgenic Linaria plants
2nd World Congress on Beneficial Microbes: Food, Pharma, Aqua & Beverages Industry
September 22-24, 2016 Phoenix, USA

Tatiana V Matveeva

St. Petersburg State University, Russia

Scientific Tracks Abstracts: Biol Med (Aligarh)

Abstract:

Most genetic engineering of plants uses Agrobacterium mediated transformation to introduce novel gene content. In nature, insertion of T-DNA in the plant genome and its subsequent transfer via sexual reproduction has been shown in several species in the genera Nicotiana, Ipomea and Linaria. They are called naturally transgenic plant. A sequence homologous to the T-DNA of the Ri plasmid of Agrobacterium rhizogenes was found in the genome of untransformed Nicotiana glauca about 30 years ago and was named �??cellular T-DNA�?� (cT-DNA). A similar cT-DNA has also been found in other species of the genus Nicotiana. In our study T-DNA-like sequences has been detected and characterized in different Linaria species, belonging to two sections Linaria and Speciosa. In all characterized Linaria species the cT-DNA is present in two copies and organized as a tandem imperfect direct repeat and contained the same T-DNA oncogenes and the mis gene, however there are different mutations inside the T-DNA in the investigated forms. Linaria species are medicinal transgenic plants that people used for ages. Characterization of structure of T-DNA in close species of naturally transgenic plants is important, since they could be good model system for study of delayed environmental risks of GMO.

Biography :

Tatiana V Matveeva has completed her PhD and Postdoctoral studies from the Faculty of Biology, St. Petersburg State University, Russia. She is a Doctor of Biological Science, presently working as a Professor of the Department of Genetics and Biotechnology, Faculty of Biology at St. Petersburg State University, Russia. She is also a Member of the Vavilov Society of Geneticists and Breeders and Member of Russian Society of Biotechnologists.

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