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Application of polyvinylpyrrolidone-polystyrene composite nanofibers coupled with high performance liquid chromatography in the analysis of several polar molecules
International Conference and Exhibition on Advances in HPLC & Chromatography Techniques
March 14-15, 2016 London, UK
Lanling Chu and Xuejun Kang
Southeast University, China
Scientific Tracks Abstracts: Pharm Anal Acta
Abstract:
Sample preparation is a crucial and tedious segment in analytical methods. The application of nano-materials in sample pretreatment is preferable and favorable to solve this bottleneck problem. In this study, several kinds of nanofibers were fabricated by electrospinning and characterized then utilized to prepare the home made extraction cartridge to extract analytes. Herein, an example was cited that a sensitive analytical method based on packed-nanofiber solid-phase extraction and high performance liquid chromatography (PFSPE-HPLC) has been developed for determination of CAP residues in milk. The optimal nanofibers, polyvinylpyrrolidone - polystyrene (PVP-PS) composite nanofibers, were selected for extraction of the CAP after comparing nanofibers and optimizing electrospinning parameters. Furthermore, condition optimization assays on pH, ionic strength, leaching and elution solvents were conducted. Under optimal conditions, the analyte was separated very well and the blank milk did not disturb the determination, and results of method validation were preferable. Additionally, PVP-PS nanofibers were suitable for extraction of quinolones and monamines in a complicated matrix. Interestingly, only several milligrams sorbent and several microlitres elution solvent were favorable to directly extract analytes in samples. This method is simple, sensitive and cost-effective, and has potential to be applied to quantitatively analyze the concentrations of polar species in samples containing complex matrix.
Biography :
Lanling Chu is currently a PhD candidate from School of Public Health of Southeast University, China. Her major is food analysis and main research field is application of nanomaterial in the analysis of food. She has published 5 papers in reputed journals.