Oral Health and Dental Management

Antimicrobial properties of silver-chitosan nanocomposite coating on PMMA: An in vitro study

4^th Asia Pacific Congress & Expo on Dental and Oral Health

July 27-29, 2015 Brisbane, Australia

Zahra Sarabadani1, 2, Mahmoud Hemmati2, Hamed Azimi1, Hassan Bahramnia1 and Arash Mohammadi2

Posters-Accepted Abstracts: Oral Health Dent Manag

Abstract:

In the present study poly methyl methacrylate (PPMA) which is the most frequently used implant in craniofacial reconstructions, is coated by a chitosan-silver nanocomposite to provide an antibacterial modification, reduce the risk of bacterial contamination and increase the success rate of implant. Two microbiological tests (antibacterial efficacy and biofilm formation) were used to evaluate this coating against Staphylococcus aureus and Pseudomonas aeruginosa. The results of antibacterial activity via colony counts showed a decrease number of S.aureus and P. aeruginosa colonies from 106.7 and 106.3 to 0 CFU/ml respectively. Two time-points of 24 and 72 hours were considered for biofilm formation. The number of S. aureus colonies formed as for the 24 hours reduced from 106.7 to 105.2 CFU/sample and in the case of P. aeruginosa it similarly declined from 106.3 to 105.1 CFU/ sample. As regard 72 hours after colonization this decrease was from 107.5 to 106.3 and 107.4 to 106.1 CFU/sample for S. aureus and P. aeruginosa respectively. The results of our study suggested the antibacterial effect of chitosan-nAg coating on PMMA against S. aureus and P. aeruginosa, however more evaluations on synergic effect of chitosan-nAg as the coating on PMMA and other materials are needed.