Biochemistry & Analytical Biochemistry

Anti-diabetic potential of Sapium ellipticum (Hochst) Pax leaf extract in streptozotocin-induced diabetic Wistar rats

Global Congress on Biochemistry, Glycomics & Amino Acids

December 08-09, 2016 San Antonio, USA

Ighodaro O M, Akinloye O A, Ugbaja R N and Omotainse, O A

1Federal University of Agriculture, Nigeria
Lead City University, Nigeria

Scientific Tracks Abstracts: Biochem Anal Biochem

Abstract:

Ethnobotanical survey associates Sapium ellipticum (SE) with antidiabetic usage among other medicinal functions in different parts of Africa. In view of this, the effects of the plant leaf extract on fasting blood glucose (FBG), glycogen and insulin levels were investigated. SE was evaluated in streptozotocin-induced diabetic adult male Wistar rats, at 400 and 800 mg kg-1 of body weight (BW), against metformin (12 mg kg-1 BW). Treatments were done orally (p.o), twice daily at 8 h interval for 21 days. SE significantly reduced FBGL by 46.5 and 44.4 % (400 and 800 mg dosage, respectively) compared to initial diabetic values. However, the effects were significantly lower than the 72.6 % glucose reduction produced by metformin. Hepatic and skeletal muscle glycogens were observed to increase (27.06 and 12.55 % respectively) in SE-treated rats (800 mg dosage) compared to their corresponding values in diabetic control animals. Plasma and pancreatic insulin contents were also improved (31.77 and 52.34%, respectively) by SE administration. The histopathological examination of the pancreas indicates beta cells regeneration in the treated animals, particularly in diabetic rats treated with 800 mg dosage of the extract compared to the diabetic control animals and metformin group. H PLC-MS analysis of SE active fractions revealed the presence of amentoflavone, lupeol and luteolin-7-O-glucoside. The outcome of this study provides scientific basis in support of the medicinal relevance of SE and lend credence to its utilization in folk medicine for the treatment of diabetes and other oxidative stress-related ailments.

Biography :

Ighodaro O M is a Lecturer in the Department of Biochemistry, Lead City University, with research interest in Biochemical Toxicology and Medicinal Plant Research. His research drive is primarily to curtail mortality and health complications that result from herbal toxicity and wrong use of herbs in treatments of ailments/diseases in Africa. He has investigated a number of plants traditionally acclaimed to possess medicinal properties in a bid to appropriately advise and orientate individuals and communities on their proper usage. His ultimate goal is to possibly isolate new drug materials with relatively better therapeutic characteristics and less or allowable side chemistry from natural sources such as plants.

Email: ighodaro.macdonald@lcu.edu.org