Journal of Nanomedicine & Nanotechnology

AFM investigation of DNA and their interaction with MCM protein complex

7^th World Nano Conference

June 20-21, 2016 Cape Town, South Africa

Amna Abdalla Mohammed Khalid

Trieste University, Italy

Scientific Tracks Abstracts: J Nanomed Nanotechnol

Abstract:

We are interested to study helicase connected to replication process in eukaryotic: is MCM (mini chromosome maintenance) complex, six homologous MCM proteins known as MCM2-7, which form a ring that is supposed to �??load�?� onto the DNA using energy produced by ATP hydrolysis and move across unwinding the double helix. In this study we used archaeal MCM from Methanothermobacterthermautotrophicusas a model system. Our main idea is to investigate the conformational changes of the DNA deposited on a mica surface upon the interaction with MCM proteins by means of AFM imaging. Based on structural and biochemical evidences, two modes of MCM-DNA interaction have been proposed. The canonical mode where the MCM protein complex is encircling the DNA and is thus �??loaded�?� onto DNA ready for unwinding. A second mode of interaction called associated MCM, where the DNA is wrapped around the external part of the proper ring structure.

Biography :

Amna Abdalla Mohammed Khalid has completed her PhD from Trieste University (Nanotechnology school). She is currently a research fellow at Nano innovation lab in Elettra- Sincrotrone Trieste. Moreover she is Next Einstein forum ambassador of Sudan.

Email: AMNA.ABDALLAMOHAMMEDKHALID@phd.units.it