Journal of Nanomedicine & Nanotechnology

A novel, nanogram sensitive method for estimation of micro-albumin in urine

8^th World Medical Nanotechnology Congress & Expo

June 08-09, 2016 Dallas, USA

P D Gupta

Manipal University, India

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

Generally, albumin is measured in urine by semi quantitative method by dipstick method. Our newly developed method is simple one-step protein estimation method to measure proteins at nanogram level concentration without involvement of any sophisticated instruments. We have used Eosin B or Eosin Y dye, which binds to at least 5 amino acids in the protein. Therefore, this method turns out to be very sensitive compared to other existing methods. Depending on the need of sensitivity, this method can be used for protein estimation in the range of 1 mg to 1000 mg with Eosin B and 200 ng to 2 ng with Eosin Y. Urine samples of 510 patients suffering from kidney disorders were collected and protein estimation was carried out by routine dipstick method and the same samples were used for estimation of protein by Eosin Y method. Out of 510 patients, 383 turn out to be normal with dipstick method whereas by Eosin Y method, 435 turn out to be normal. Out of 510 patients on the basis of dipstick method, 127 were recommended for kidney dialysis; whereas, on the basis of Eosin Y method only 75 really needed dialysis. This method can be carried in any diagnostic laboratory equipped with a UV spectrometer.

Biography :

Email: pdg2000@hotmail.com