A low molecular weight polysaccharide isolated from Agaricus blazei inhibits expression of E-selectin in activated endothelial cells and attenuates tumor cell adhesion
2nd World Congress on Bioavailability & Bioequivalence: Pharmaceutical R & D Summit-2011 and International Conference on Pharmaceutics & Novel Drug Delivery Systems
06-08 June 2011, Las Vegas, USA

Ji-cheng Liu, Liling Yue

Scientific Tracks Abstracts: PAA

Abstract:

E-selectin-mediated cell-cell adhesion plays an important role in infl ammatory processes and extravasation of tumor cells. Tumor necrosis factor-α (TNF-α) induces E-selectin gene and protein expression in human umbilical vein endothelial cells (HUVEC). In the present study, we show a low molecular weight polysaccharide isolated from Agaricus blazei (LMPAB) depress the TNF-α-stimulated increase in E-selectin protein expression, and similar results were obtained for E-selectin mRNA expression. Th e results indicated that the eff ect of LMPAB is based on inhibition of gene expression, and the eff ective inhibitory of LMPAB was in a dose-dependent manner. In addition, LMPAB depress n uclear factor -kappa B protein expression and nuclear translocation. Inhibition of E-selectin expression by LMPAB gives rise to a signifi cant reduction in TNF-α stimulated adhesion of HT-29 cells to HUVEC. Th e data support the view that LMPAB might be therapeutic agent against E-selectin- mediated metastasis.

Biography :

Ji-Chegn Liu has completed his Ph.D at the age of 48 years from Beijing university of Chinese medicine and postdoctoral studies from Harbin medical University. He is the president of Qiqihar medical university. He has published more than 80 papers in reputed journals and serving as an editorial board member of repute.