Journal of Nanomedicine & Nanotechnology

A DNA tinker-toy for cancer-specific RNA detection

8^th World Medical Nanotechnology Congress & Expo

June 08-09, 2016 Dallas, USA

Woo Jung Shin, Seung Won Shin and Soong Ho Um

Sungkyunkwan University, South Korea

Posters & Accepted Abstracts: J Nanomed Nanotechnol

Abstract:

With the emergence of a DNA nanotechnology, a variety of DNA materials have been manufactured; they included a DNA dendrimer, a polyhedral DNA and even DNA emoticons. They have been practically applied into current biodiagnostic fields because of the intrinsic biocompatibility and specific selectivity. Here, simple DNA materials were transformed into multifunctional complexes as equipped with functional modules such as environment-responsive nanosensors. Inspired by these achievements, we developed a hairpin-looped Y-shaped DNA construct, in which there are several sensing probes and these modules are specialized without a constraint. It was simply named a DNA tinker-toy. The DNA tinker-toy briefly comprised of two functional components for effective cancer-specific RNA detection: (1) A DNA scaffold made of Y-DNA and (2) A DNA capture identifying a cancer-specific RNA sequence. The new DNA tinker-toy was thoroughly evaluated and optimized under several conditions. It was confirmed by observing a retarded band shift in the gel electrophoresis because of its higher molecular weight after total combinative synthesis. In cancer diagnosis, it is highly specific to several RNA markers originated from breast cancer model and rapidly detected via a fluorescence resonance enhanced transfer. It is speculated that the DNA tinker-toy may be a promising in situ cancer diagnostic tool.

Biography :

Email: wj.shin@skku.edu