Mechanism of the Introduction of Exogenous Genes into Cultured Cells Using DEAE-Dextran-MMA Graft Copolymer as a Non-Viral Gene Carrier. II. Its Thixotropy Property

Yuki Eshita^1*, Junko Higashihara¹, Masayasu Onishi², Masaaki Mizuno³, Jun Yoshida⁴, Tomohiko Takasaki⁵, Hidekatsu Yoshioka⁶, Naoji Kubota⁷ and Yasuhiko Onishi^2*

¹Department of Infectious Disease Control, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan

²Ryujyu Science Corporation, 39-4 Kosora-cho, Seto-shi, Aichi Prefecture 489-0842, Japan

³The Center for Genetic and Regenerative Medicine, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho, Showa-ku, Nagoya-shi, Aichi Prefecture 466- 8550, Japan

⁴Department of Neurosurgery, Graduate School of Medicine, Nagoya University, ,65 Tsurumai-cho, Showa-ku, Nagoya-shi, AichiPrefecture466-8550, Japan

⁵Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640, Japan

⁶Department of Matrix Medicine, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan

⁷Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu-shi, Oita Prefecture 879-5593, Japan